https://doi.org/10.1051/epjconf/20134107013
Measuring enzyme binding using shaped ultrafast laser pulses
1 Department of Physics and Astronomy, Dickinson College, Carlisle, PA, 17013, USA
2 Department of Physics and Astronomy, Stony Brook University, Stony Brook, NY, 11794, USA
We use multiphoton quantum-control spectroscopy to discriminate between enzyme-bound and unbound NADH (reduced nicotinamide adenine dinucleotide) molecules in solution. Shaped ultrafast laser pulses are used to illuminate both forms of NADH, and the ratio of the fluorescence from the bound and unbound molecules for different pulse shapes allows us to measure binding without spectrally resolving the emitted fluorescence or relying on the absolute fluorescence yield. This permits determination of enzyme binding in situations where spectrally resolved measurements and absolute fluorescence yields are difficult to obtain, and makes the approach ideal for multiphoton microscopy with molecular discrimination.
© Owned by the authors, published by EDP Sciences, 2013
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 2.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
