Pulse-shaping-based two-photon FRET microscopy
1 Applied Physics Program, University of Michigan, 450 Church Street, Ann Arbor, MI, USA 48109
2 Department of Physics and Biophysics, University of Michigan, 450 Church Street, Ann Arbor, MI, USA 48109
3 Department of Microbiology and Immunology, University of Michigan Medical School, 1150 West Medical Center Drive, Ann Arbor, MI, USA 48109
We present quantitative pulse-shaping-based two-photon fluorescence resonance energy transfer microscopy. We tailor the spectral phase of the excitation pulses to achieve selective excitation of donor and acceptor, demonstrating the method in live cells.
© Owned by the authors, published by EDP Sciences, 2013
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 2.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.