Single Donor-Acceptor Pairs as a Tool for Studying Conformational Dynamics of Proteins and Other Macromolecules

I.S. Osad’ko

doi:10.1051/epjconf/201510305005

EPJ

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Proceedings

Open Access

EPJ Web of Conferences 103, 05005 (2015)
https://doi.org/10.1051/epjconf/201510305005

Single Donor-Acceptor Pairs as a Tool for Studying Conformational Dynamics of Proteins and Other Macromolecules

I.S. Osad’ko^a

Institute for spectroscopy of RAS, 142190, Troitsk, Moscow, Fizicheskaya 5

^a Corresponding author: osadko@isan.troitsk.ru

Published online: 25 September 2015

Abstract

Influence of triplet states in donor and acceptor molecules on function E(R_DA) describing dependence of FRET efficiency E on inter-dye distance R_DA is considered. Formula for E(R_DA) differs from conventional equation used widely in practice and it shows that triplet states hampers energy transfer in D-A pair, considerably.

Key words: single donor-acceptor pair / efficiency of energy transfer

© Owned by the authors, published by EDP Sciences, 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.