Single Donor-Acceptor Pairs as a Tool for Studying Conformational Dynamics of Proteins and Other Macromolecules
Institute for spectroscopy of RAS, 142190, Troitsk, Moscow, Fizicheskaya 5
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Published online: 25 September 2015
Influence of triplet states in donor and acceptor molecules on function E(RDA) describing dependence of FRET efficiency E on inter-dye distance RDA is considered. Formula for E(RDA) differs from conventional equation used widely in practice and it shows that triplet states hampers energy transfer in D-A pair, considerably.
Key words: single donor-acceptor pair / efficiency of energy transfer
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