https://doi.org/10.1051/epjconf/201713305001
Surface-enhanced Raman scattering and Plasmon effect for enzymatic bionanocomplexes characterization
1 Centre for Microelectronics and Nanotechnology, University of Rzeszow, S. Pigonia 1, 35-959 Rzeszow, Poland
2 Department of Biotechnology, Institute of Applied Biotechnology and Basic Sciences, University of Rzeszow, Werynia 502, 36-100 Kolbuszowa, Poland
3 Faculty of Mathematics and Natural Sciences, University of Rzeszow, S. Pigonia 1, 35-959 Rzeszow, Poland
4 Institute of Metallurgy and Materials Science Polish Academy of Sciences, Reymonta 25, 30-059 Krakow, Poland
5 Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov 14/16, Lviv 79005, Ukraine
* Corresponding author: rwojnar@ur.edu.pl
Published online: 15 December 2016
Cholesterol oxidase (ChOX) enzyme is one of the most important analytical enzyme, used for cholesterol assay in clinical diagnostics as well as food production, and the developing of innovative solutions for improving the selectivity and accuracy of the analysis including bio-nanotechnological approaches is still ongoing. The Surface Plazmon Resonance (SPR) and the surface enhanced Raman scattering (SERS) as specific for nanocurriers effects were observed what enable us to research the oscillation spectra of the ChOX enzyme. The vibrational lines are attributed to chemical functional groups existing in enzyme, for example, amino acids, amide groups as well as for cofactor. For the improving the SERS effect the gold nanoparticles – ChOX bionanocomplexes were analyzed in combination with gold-coating gratings as a promising plazmonic material.
© The Authors, published by EDP Sciences, 2017
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
