https://doi.org/10.1051/epjconf/201819003008
Live-cell nanoscopy enabled with transient labeling and the control of fluorophore blinking
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences 16/10 Miklukho-Maklaya, Moscow, 117997, Russian Federation
* Corresponding author: mishin@ibch.ru
Published online: 26 September 2018
Live-cell super-resolution of proteins labeled with genetically encoded fluorescent tags is a challenging task because of the imperfect labeling and the inevitable deterioration of the signal in the course of the experiment. Incomplete maturation of the covalently attached fluorescent tags, inefficient photoconversion, and photobleaching further complicate prolonged live-cell nanoscopy. We have implemented two strategies for lowering the photodamage: ensuring the dynamic replacement of damaged molecules and establishing conditions for the robust intrinsic blinking of the tags at lower illumination powers.
© The Authors, published by EDP Sciences, 2018
This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
