https://doi.org/10.1051/epjconf/202125506003
Stimulated Raman Microscopy Implemented by Three Femtosecond Laser Sources
1 CHT @Erzelli, Nanoscopy, Istituto Italiano Di Tecnologia, Genova, Italy
2 National Research Council (CNR), Institute of Applied Sciences and Intelligent Systems, Napoli, Italy, Napoli, Italy
* Corresponding author: rajeev.ranjan@iit.it
Published online: 18 November 2021
In this work, the implementation of a femtosecond Stimulated Raman Scattering microscope, equipped with three femtosecond laser sources: a Titanium-Sapphire (Ti:Sa), an optical parametric oscillator (OPO), and a second harmonic generator (SHG); is presented. Our microscope is designed so that it can cover all the regions of Raman spectra, taking advantage of two possible laser combinations. The first, Ti:Sa and OPO laser beams, which cover the C-H region (>2800 cm-1 ) in stimulated Raman gain (SRG) modality, whereas the second, Ti:Sa and SHG laser beams, covering the C-H region and the fingerprint region in stimulated Raman losses (SRL) modality. The successful realization of the microscope is demonstrated, reporting images of polystyrene beads using both SRL and SRG modalities.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
