https://doi.org/10.1051/epjconf/202125511009
A Spectral Resolution study in Femtosecond Stimulated Raman Scattering Microscopy
1 National Research Council (CNR), Institute of Applied Sciences and Intelligent Systems, Napoli, Italy
2 CHT @Erzelli, Nanoscopy Istituto Italiano Di Tecnologia, Genova, Italy
* Corresponding author: rajeev.ranjan@iit.it
Published online: 18 November 2021
The simultaneous mapping and the specificity of different chemical species are desirable in several biological and biomedical applications. The stimulated Raman Scattering technique is a proven and well-established label-free method to map the distributions of individual species in a multi-component-based system due to the linear dependence of signals on concentration and its chemical selectivity. In this framework, spectral resolution, i.e., the ability to distinguish closely lying resonances, plays a fundamental role. Here in this work, the cross-correlation of Ti:Sa & OPO femtosecond laser beams in a stimulated Raman scattering microscope is measured. The separation between protein and lipid bands in the C-H region is important for biochemical research and is successfully classified.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
