https://doi.org/10.1051/epjconf/202125513003
Optical multiplexed bioassays on photonic crystals for breast cancer biomarker detection
1 Department of Basic and Applied Science for Engineering, Sapienza University of Rome, Via A. Scarpa 16, 00161, Rome, Italy.
2 IRCCS Regina Elena National Cancer Institute, Via Elio Chianesi 53, 00144 Rome, Italy.
3 Fraunhofer Institute for Applied Optics and Engineering IOF, A. Einstein-Str. 7, 07745 Jena, Germany.
4 Fraunhofer Institute for Material and Beam Technology IWS, Winterberg-Str. 28, 01277 Dresden, Germany.
* Corresponding author: tommaso.pileri@uniroma1.it
Published online: 18 November 2021
An optical biosensor for proteomic breast cancer biomarker detection in complex media is presented. Bloch Surface Waves (BSW) excited onto one dimensional photonic crystal (1DPC) were used to probe the interaction of HER2 with three antibody species and an inert protein (Bovine Serum Albumin - BSA). The optical system combines Label-Free readings to track the bioassay real-time development and Fluorescence emission quantification to evaluate the level of specific interaction between the antigen and the antibodies. The results confirm a distinguishable level of affinity between the antibodies and the analyte according to their specificity even at low antibody surface density (about 1173 pg/mm2).
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
