https://doi.org/10.1051/epjconf/202226604004
Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow-driven samples in a light-sheet fluorescence microscope
1 Technische Universität Ilmenau, Fachgebiet Technische Optik, Postfach 100565, 98684 Ilmenau, Germany
2 Institut für Bioprozess- und Analysenmesstechnik e.V., Rosenhof, 37308 Heilbad Heiligenstadt, Germany
* e-mail: meike.hofmann@tu-ilmenau.de
Published online: 13 October 2022
Light-sheet fluorescence microscopy (LSFM) with single light-sheet illumination enables rapid 3Dimaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets along an inclined tube for applications in flow-driven imaging. The element, which is based on a multi-focal Fresnel zone plate and a linear grating, generates in combination with a refractive cylindrical lens five thin light sheets of equal intensity.
© The Authors, published by EDP Sciences
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