https://doi.org/10.1051/epjconf/202328703016
Multispectral imaging via feature selection: A frugal innovation approach for pathogen identification
1 Technology Research on Infectious Diseases | Open Innovation & Partnerships Dept., bioMérieux, Marcy l’Etoile, France
2 R&D Microbiology, bioMérieux, La Balme Les Grottes, France
* Corresponding author: denis.leroux@biomerieux.com
Published online: 18 October 2023
In order to develop an affordable clinical diagnostic instrument for use in more decentralized settings, we have assessed the feasibility to move from hyperspectral to multispectral imaging via parsimonious feature selection. The targeted application is the label-free identification at the species-level of uropathogens from images of bacterial colonies on their growth support. We show that the number of predictors (i.e., discrete spectral channels), can be dramatically reduced from 240 to less than 10 channels with limited performance loss. The impact of bandwidth is also investigated to consider the high degree of redundancy of raster images obtained by diffuse reflectance and propose a suitable design for a simple filter wheel based solution. Targeting the 8 most prevalent bacterial species responsible for > 80% of urinary tract infections, up to 94% of correct identification rate was reached using only 4 spectral windows.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
