https://doi.org/10.1051/epjconf/202328703018
Broadband CARS high-throughput single-cell imaging
1 Department of Electronic Engineering, Maynooth University, Co. Kildare, Ireland
2 Department of Computer Science, Maynooth University, Maynooth, Co. Kildare, Ireland
* e-mail: ryan.muddiman.2021@mumail.ie
** e-mail: bryan.hennelly@mu.ie
Published online: 18 October 2023
Broadband Coherent anti-Stokes Raman Scattering (BCARS) enables the whole vibrational spectrum of cytologically prepared samples to be obtained using a hyperspectral raster scan approach. This technique has the potential to enable high-throughput automated detection of cell abnormalities. Images are distorted by the non-resonant background which requires a treatment for proper analysis. Using statistical denoising and phase retrieval returns Raman spectra similar to that of a spontaneous Raman measurement. Here, we present our work using this method for single-cell imaging of PEO1 ovarian adenocarcinoma cells prepared with the ThinPrep processor which enables label-free Raman cytology.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
