https://doi.org/10.1051/epjconf/202328709015
Microstructured molecular BIO-gratings by means of UV induced denaturation - INVITED
1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, 46022 Valencia, Spain
2 Laboratory of Fiber Optics - Institut de Ciència dels Materials (ICMUV), Universitat de València, Catedrático José Beltrán 2, 46980 Paterna,Valencia, Spain
3 Departament de Química, Universitat Politècnica de València, 46022 Valencia, Spain.
* Corresponding author: martina.delgado@uv.es
Published online: 18 October 2023
Rapid, reliable and low cost techniques to fabricate biosensors is a hot topic nowadays. Here, we present a BIO-grating fabricated by means of local, selective denaturing of molecules using UV radiation. A phase-mask is used to generate an interferometric pattern of 1420 nm pitch that, when illuminating a biolayer of BSA molecules lead to its periodic deactivation. After the biorecognition of the specific antibody, aBSA, a BIO-grating is generated due to the height difference between the protein, and the complex protein + antibody. We present the optimization of the fabrication of the BIO-gratings and their AFM characterization. Also, the biosensor performance in terms of limit of detection and limit of quantification will be presented.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
