https://doi.org/10.1051/epjconf/202430904006
Development of a Microfluidic Device for Blood Cells Extraction in Liquid Biopsy
1 Dipartimento Interateneo di Fisica Michelangelo Merlin, University & Polytechnic of Bari, 70126 Via Giovanni Amendola 173, Italy
2 Universitat Politecnica de Catalunya (UPC), 08222 Campus de Terrassa Carrer de Colom 1, Spain
3 CNR-IFN UOS BARI, Via Amendola 173, 70125 Bari, Italy
4 CNR-IFN UOS Milano, Piazza Leonardo da Vinci 32, 20133 Milan, Italy
* Corresponding author: caragnanostefania@gmail.com
Published online: 31 October 2024
This project aims to produce a microfluidic device capable of separating 6 μm and 20 μm diameters particles by inertial sorting. This Lab-on-Chip (LoC) was designed with a trapezoidal cross-section for better fluid control and effective particle manipulation at the microscopic level, as demonstrated by COMSOL simulations. The device was manufactured on a substrate of Polymethyl Methacrylate (PMMA) by femtosecond laser technology and then assembled using an innovative geometry-preserving Isopropyl alcohol-based procedure. The LoC was test with spherical plastic microparticles of two diameters (6 μm and 20 μm) suspended in distilled water. The separation efficiencies were (98.2 ± 1.6) % for 20 μm diameter particles and (70.0 ± 1.8) % for 6 μm diameter particles in good agreement with the simulation results. Finally, after a microfluidic channels’ acetone vapors treatment, the device demonstrated a good ability to separate biological particles (Red Blood Cells) at different concentrations (20%, 30%, 40%, 50%) in a PBS buffer.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
