https://doi.org/10.1051/epjconf/202430904019
Porous Silicon modified by Molecularly Imprinted Polymers for Interleukin-6 (IL-6) detection
1 Department of Engineering (DI), University of Naples Parthenope, Centro Direzionale Isola (C4), 80134, Naples (Italy)
2 Institute of Applied Sciences and Intelligent Systems (ISASI), National Research Council of Italy (CNR), Naples, Italy
3 Institute of Nanotechnology, CNR-Nanotec, Via Monteroni, 73100, Lecce, Italy
* Corresponding author: luca.destefano@na.isasi.cnr.it
Published online: 31 October 2024
In this study, we present an innovative optical biosensor designed for the precise detection of Interleukin-6 (IL-6), a crucial cytokine associated with various pathological conditions. Our biosensor is based on silicon porous material meticulously modified with a molecularly imprinted polymer (MIP), ensuring specific and sensitive recognition of IL-6 molecules. Fabrication process involves the electrochemical etching of silicon porous chips followed by the electrodeposition of MIP, tailored to selectively bind IL-6 targets. Through rigorous testing across a range of IL-6 concentrations, our sensor exhibits remarkable sensitivity, showcasing discernible optical responses proportional to the varying analyte concentrations. Furthermore, we assessed the sensor's performance using bovine serum, a complex biological matrix, to simulate real-world sample conditions. Encouragingly, the sensor maintains its selectivity and optical response in the presence of serum components, affirming its robustness and applicability in practical diagnostic settings.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
