https://doi.org/10.1051/epjconf/202430910007
A sensorised, microfluidic Ussing chamber manufactured by stereolithography and soft lithography techniques
1. Photonics4Life research group, Department of Applied Physics, Facultade de Física and Institute of Materials (iMATUS), Universidade de Santiago de Compostela, Campus Vida, Santiago de Compostela, 15782, Spain
2. BFlow S.L., Edificio Emprendia, Santiago de Compostela, 15706, Spain
3. International Iberian Nanotechnology Laboratory (INL), Avenida Mestre José Veiga s/n. 4715-330 Braga, Portugal
* Corresponding author: maite.flores@usc.es
Published online: 31 October 2024
Motivated by the need for improved platforms in biomedical research, this study addresses challenges associated with traditional Ussing chamber systems, widely used in studying biological barriers like the epithelial barrier of the gut. These challenges include complexity, high sample volumes, and limited compatibility. By combining stereolithography and soft lithography techniques, a microfluidic Ussing chamber is manufactured, overcoming these limitations, and incorporating compatibility with microscopy. Validation through Trans-Epithelial Electrical Resistance (TEER) measurements confirms its efficacy in assessing ion permeability dynamics, utilizing Caco-2 cell monolayers. The study showcases the capability of the manufactured chamber for sensing impact of calcium on tight junctions.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
