https://doi.org/10.1051/epjconf/201921510003
Microfluidic engineering for continuous in-flow cyto-tomography
1
National Research Council of Italy, Institute of Applied Sciences and Intelligent Systems “E. Caianiello”, Via Campi Flegrei 34, Pozzuoli, Naples, Italy.
2
Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, University of Naples “Federico II”, Piazzale Tecchio 80, 80125 Napoli, Italy.
* Corresponding author: p.memmolo@isasi.cnr.it
Published online: 10 September 2019
The possibility to investigate cells in microfluidic flow by using a full 3D morphometry analysis is highly demanded to achieve information about their healthiness. Recently, the tomographic flow cytometry by digital holography has been demonstrated to monitor red blood cells in microfluidics environment by simply applying flux pressure to induce random self-rotation of flowing cells. Here, we provide a microfluidic solution to engineer the flow with the aim to ensure the full 360 degree of angle rotation of all cells in the field of view. We test the proposed methods for circulating tumour cells.
© The Authors, published by EDP Sciences, 2019
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
