https://doi.org/10.1051/epjconf/201921511002
Optofluidic lab-on-chips for high throughput 3D imaging of cells and tissues
1
Istituto di Fotonica e Nanotecnologie - Consiglio Nazionale delle Ricerche (IFN-CNR), 20133 Milano, Italy
2
Dipartimento di Fisica - Politecnico di Milano, 20133 Milano, Italy
* Corresponding author: francesca.bragheri@ifn.cnr.it
Published online: 10 September 2019
Optofludic lab-on-chips are compact microsystems that allow the manipulation, the analysis and the imaging of cells with high throughput even at the single cell level. The optofluidic microscopes on chip here presented are fabricated by femtosecond laser micromachining followed by chemical etching. The technique is ideally suited for rapid prototyping and allows 3D geometries and perfect alignment between microfluidic and optical components networks. The devices have been validated on cancer cell lines by imaging in three dimensions both clusters of cells growing on spheres and single cells.
© The Authors, published by EDP Sciences, 2019
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
