https://doi.org/10.1051/epjconf/202328703005
Fast Adaptive Optics in optically sectioned fluorescence microscopes for functional neuroimaging
1 Imagine Optic, 91400 Orsay, France
2 Institut des Sciences Moléculaires d’Orsay (ISMO), 91400 Orsay, France
3 Ecole Normale Supérieure – Institut de Biologie (ENS-IBENS), 75005 Paris, France
* Corresponding author: fharms@imagine-optic.com
Published online: 18 October 2023
We demonstrate how a novel approach for closed-loop Adaptive Optics (AO) specifically adapted to microscopy enables straightforward integration in Light-Sheet and Multiphoton microscopes, as well as fast aberration correction. We present corresponding experimental setups as well as first demonstrations of the benefits of the correction of sample-induced aberrations in zebrafish and mouse brain tissue, with the ultimate goal to enable high-speed, high-sensitivity functional imaging at large depths.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
