https://doi.org/10.1051/epjconf/202328703014
Assessing embryo quality with digital holographic microscopy
1 SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, Fife, United Kingdom
2 Robinson Research Institute, School of Biomedicine, The University of Adelaide, Adelaide, Australia
3 Centre of Light for Life and School of Biological Sciences, The University of Adelaide, Adelaide, Australia
* Corresponding author: god1@st-andrews.ac.uk
Published online: 18 October 2023
A low-powered, non-invasive digital holographic microscopic imaging technique for embryo quality assessment is presented. Two groups of 2 cell-stage embryos cultured in media of different lipid concentrations have been characterized with digital holographic microscopy for lipid aggregation. The study suggests refractive index measurements are reflective of the lipid and dry mass content in embryos thus making DHM a prospective label-free diagnostic tool for quality assessments in assisted reproduction.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
