Proceedings

EPJ TI Review - Applications of Raman micro-spectroscopy to stem cell technology

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This short review, part of the Thematic Series on Raman Spectroscopies, describes the application of Raman micro-spectroscopy to measure the molecular properties of stem cells during differentiation in-vitro. The paper focuses on time- and spatially-resolved Raman spectral measurements that allow repeated investigation of live stem cells in-vitro.

Stem cell therapy is widely acknowledged as a key medical technology of the 21st century which may provide treatments for many currently incurable diseases. These cells have an enormous potential for cell replacement therapies for curing diseases such as Parkinson’s disease, diabetes and cardiovascular disorders, as well as in tissue engineering as a reliable cell source for providing grafts to replace and repair diseased tissues. Nevertheless, the progress in this field has been difficult in part because of lack of techniques that can measure non-invasively the molecular properties of cells. Raman spectroscopy is an optical technique based on inelastic scattering of laser photons by molecular vibrations of cellular molecules and can be used to provide chemical fingerprints of cells or organelles without fixation, lysis or use of labels and other contrast enhancing chemicals. Because differentiated cells are specialized to perform specific functions, these cells produce specific biochemicals that can be detected by Raman micro-spectroscopy.

I thank you very much for all suggestion and help during the various steps of the procedure and contacts regarding the preparation of documents, articles and information for the publication of the DHF 2014 Conference proceedings in EPJ Web of Conferences vol.96 (2015) of the EDP sciences publisher. It is the second time in the last two years that we decided to choose your journal for the proceedings publication of our conference and symposium, and we are glad for the experience had with your staff and also for the importance of your journal in order to support our conferences and for the dissemination of our scientific activity. I am also very glad for the prompt publication and it is an honour for our organization to have such a publication on your journal.

Giorgio Giardina, Messina University - INFN Catania, Italy
Scientific Secretary of the MESON Conference series, EPJ Web of Conferences vol. 96, 2015

ISSN: 2100-014X (Electronic Edition)

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