https://doi.org/10.1051/epjconf/202328703011
Volumetric one-photon UVA hyperspectral light sheet imaging in mouse pre-implantation embryos - INVITED
1 SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, Fife, United Kingdom
2 Robinson Research Institute, School of Biomedicine, The University of Adelaide, Adelaide, Australia
3 Centre of Light for Life and School of Biological Sciences, The University of Adelaide, Adelaide, 5005, Australia
* Corresponding author: jmm46@st-andrews.ac.uk
Published online: 18 October 2023
UVA one-photon hyperspectral light sheet imaging was performed here to capture and reconstruct three dimensional metabolic maps of mouse embryos during development. We explored numerically and experimentally the advantages of phasor-based hyperspectral approach for detecting embryo metabolism with a single wavelength excitation compared to the conventional detection approach using bandpass filters.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
