https://doi.org/10.1051/epjconf/202328703012
Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy
1 Politecnico di Milano, Dipartimento di Fisica, 20133 Milano, Italy
2 Università degli Studi di Milano, Dipartimento di Bioscienze, 20133 Milan, Italy
* Corresponding author: andrea1.bassi@polimi.it
Published online: 18 October 2023
Root hairs are a delicate single-cell system whose growth is regulated by a fine mechanism characterised by the presence of a tip-high Ca2+ gradient that shows regular oscillations in growing root hairs. We show a method based on the use of Light sheet fluorescence microscopy (LSFM) which allows the quasi-physiological analysis of Arabidopsis thaliana plant roots hairs with excellent spatial and temporal resolution over a wide field of view. We show how the healthy growing root hairs are linked to precise oscillations and how a disruption of this mechanism can be associated to specific genes.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
